Question

A small fluorescent object lies just inside at the bottom of a nearly spherical cell. The cell is 100 µm in diameter and has an index of refraction of 1.37. The cell lies in a 200 µm deep culture medium of refractive index 1.33. How deep does the fluorescent object appear to be when viewed through the culture medium and the cell body?

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Answer #1

Ray NOmal cultime medium 2123 ISOLM 50um Sobject Imege(T leiginatadmte Ghjet Consider as fasser ugh ceuto c so t fases WHoi33/137) (1-33/2)- 137 (-50) 50 C-33-1-37) 1-33 1:312 +L So -SOX1-37 133 1.3722 - O.O4 50x137 0-04-137 SOX137 50 1.33 12T1 (33

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