Question

1) If a culture has an instantaneous growth rate constant of 0.00090832 min-1, what is the...

1) If a culture has an instantaneous growth rate constant of 0.00090832 min-1, what is the doubling time of the culture?

  • Express your answer in minutes using exponential notation.
    • 48.2 minutes would be 4.82E1
  • Round your answer to two decimal places
    • 32.67 minutes would be 3.27E1

2)Given a doubling time of 635 minutes, how long will it take a culture to grow from 14 cells per mL, to 47,621 cells per mL?

  • Report your answer in minutes, rounded to 2 decimal places.
  • Do not use exponential notation.
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Answer #1

Answer 1:)

Calculate the doubling time by use the following formula:

Td = 70/r

Td is the doubling time, and r is the growth rate.

Given that r = 0.00090832 min-1

Td = 70/0.00090832

Td = 77065.35

Td = 7.7E4 mins

Answer 2:)

To calculate the time of the growth of a culture, use the following formula:

Nt = N02t/Td

Nt = number of cells (t time).

N0 is the initial number of cells.

Given that,

Nt = 47,621 cells per mL

N0 = 14 cells per mL

Td = 635 minutes

47621 = 14 x 2t/635

2t/635 = 3401.5

Taking log on both sides

Log 2t/635 = log 3401.5

t/635 x log2 = 3.53

t/635 x 0.3 = 3.53

t = (3.53/0.3) x 635

t = 0.00167 minutes

t = 1.67 x 10 -3 minutes

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