1. Using your understanding of PCR amplification answer the following questions:
a) How do you know if a primer dimer is created during PCR?
b) What is nonspecific PCR amplification and how do you know if it exists during PCR?
c) What are the possible results you could expect to observe on the agarose gel from your PV92C results? Use a schematic diagram and draw a gel to illustrate where the PV92 primers bind. You should also consider where the primers bind on the paternal and maternal chromosomes as well.
Answer1)- A):-
:- Primer Dimer is a product of PCR that is formed when the complementary strands of primer DNA binds with each other.
It can be recognised by the following methods:-
(According to the Chegg guidelines I am unable to answer all the questions, hope you understood this one very well !)
1. Using your understanding of PCR amplification answer the following questions: a) How do you know...
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You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA as the template. Assume there are no primer-dimer formations or non-specific priming events. What banding patterns could you possibly see on the gel after you complete the PCR reaction? a) 1 band only (INCORRECT) b) 2 bands c) 3 bands d) a and b are possible e) all of the above are possible f) impossible to predict
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