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You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA...

You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA as the template. Assume there are no primer-dimer formations or non-specific priming events. What banding patterns could you possibly see on the gel after you complete the PCR reaction?

a) 1 band only

b) 2 bands

c) 3 bands

d) a and b are possible

e) all of the above are possible

f) impossible to predict

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Answer #1

As there in no primer dimer formations and no nonnosoecific binding and one locus will have one particular sequences against which the primer had been used that's why the primer will bind the sequences of that particular DNA strand..

And will amplify the allele which is particular for that primers .

The amount of DnA will also be increased and when you will run the agarose gel it will give you one band only.

Thank you

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