Compare the strength of the following promoters for the sigma 70 RNA Polymerase subunit: ...
Compare the strength of the following promoters for the sigma 70 RNA Polymerase subunit:
-35 -10 +1
1. TTGACA TATAAT A
2. CGTACC TTTAAA G
3. TTCACC TCTAAT A
4. ATGACC TACAAT C
5. TAGAAC AATATT A
Arrange the numbers for the promoters from strongest to weakest.
Homework Answers
Following is the order from strongest to weakest.
1. For this sequence -35 and -10 sequences are the typical consensus sequence which can be recognized by Sigma factor of RNA polymerase followed by 3, 5, 4 and 2.
This has been deciphered on the basis of decreasing number of nucleotides match with that of the reference consensus sequence.
Add Answer to:
Compare the strength of the following
promoters for the sigma 70 RNA Polymerase subunit:
...
Part A The sigma (?) subunit binds to the core RNA polymerase enzyme. The function of...
Part
A
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to the promoter of a gene so that transcription can be initiated.
The closeup shows the secondary structure of the sigma (?)
subunit, which consists of four domains. Identify the domains
labeled 1-3.
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to...
4. A promoter for an E. coli gene that is transcribed by a s-70 RNA polymerase...
4. A promoter for an E. coli gene that is transcribed by a s-70 RNA polymerase has the following sequence: 30 -20 10 +1 5'GGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA 3'CCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCT The transcription start site +1) is identified. a. Identify the -10 and -35 sequences. How close are they to the consensus-10 and-35 sequences? b. What is the spacing between the -10 and the -35 sequences? How does this compare with the consensus spacing? C. The sequence of bases in a transcribed RNA is identical...
4. Answer the following concerning promoters and transcription. (15 points) a. What role do promoters play...
4. Answer the following concerning promoters and transcription. (15 points) a. What role do promoters play in transcription? b. What is the common structure of a bacterial promoter with respect to its consensus sequences? c. What is the role of the sigma subunit for bacterial RNA polymerase as it relates to promoters? d. Eukaryotic promoters are more variable than prokaryotic promoters. Explain why e. Explain what would happen to a eukaryotic promoter if it were associated with acetylated
Below is a diagram of a transcription unit and the mRNA made from the transcription unit:...
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: А H DNA TTGACA TATAAT -35 B -10 C D Start codon Stop codon mRNA 5 E F G Is this a prokaryotic or eukaryotic transcription unit? What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoters Pribnow box Non-template strand Transcriptional start site- Open Reading...
2. The sequences of 13 promoters recognized by σ70 factor of RNA polymerase have been aligned...
2. The sequences of 13 promoters recognized by σ70 factor of RNA
polymerase have been aligned below. Deduce the consensus sequences
for the -10 and -35 regions of these promoters. The TSS is
highlighted. Do your consensus sequences agree with the actual
consensus sequences? If not, why?
Gene 1: TCTCAACGTAACACTTTACAGCGGCG--CGTCATTTGATATGATGC-GCCCCGCTTCCCGATAAGGG Gene 2: GATCAAAAAAATACTTGTGCAAAAAA--TTGGGATCCCTATAATGCGCCTCCGTTGAGACGACAACG Gene 3: ATGCATTTTTCCGCTTGTCTTCCTGA--GCCGACTCCCTATAATGCGCCTCCATCGACACGGCGGAT Gene 4: CCTCAAATTCAGGGTTGACTCTGAAA--GAGGAAAGCGTAATATAC-GCCACCTCGCGACAGTGAGC Gene 5: CTGCAATTTTTCTATTGCGGCCTGCG--GAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGAT Gene 6: TTTTAAATTTCCTCTTGTCAGGCCGG--AATAACTCCCTATAATGCGCCACCACTGACACGGAACAA Gene 7: GCAAAAATAAATGCTTGACTCTGTAG--CGGGAAGGCGTATTATGC-ACACCCCGCGCCGCTGAGAA Gene 8: TAACACCGTGCGTGTTGACTATTTTA-CCTCTGGCGGTGATAATGG--TTGCATGTACTAAGGAGGT Gene 9: TATCTCTGGCGGTGTTGACATAAATA-CCACTGGCGGTGATACTGA--GCACATCAGCAGGACGCAC Gene 10: GTGAAACAAAACGGTTGACAACATGA-AGTAAACACGGTACGATGT-ACCACATGAAACGACAGTGA Gene 11: TATCAAAAAGAGTATTGACTTAAAGT-CTAACCTATAGGATACTTA-CAGCCATCGAGAGGGACACG...
which of the following regarding th sigma factor of E.coil RNA polymerase is correct? a) is...
which of the following regarding th sigma factor of E.coil RNA polymerase is correct? a) is required for termination of an RNA chain b is inseparable from the core enzyme c) will analyse synthesis of RNA from both strands of the DNA template in the absence of the d) combines the core enzyme to confer specific binding to a promoter q2 which of the following is not true of all naturally occurring DNA 1) two hydrogen bonds from A and...
Arrange the following oxoacids in order of decreasing acid strength.
Part A Arrange the following oxoacids in order of decreasing acid strength.Rank from strongest to weakest acid. To rank items as equivalent, overlap them.1. HClO22. HClO33. HBrO4. HClOPart B Arrange the following carboxylic acids in order of decreasing acid strength.1. CHCl2COOH2. CH2ClCOOH3. CH3COOH4. CH3CH2COOHPart C Arrange the following amines in order of decreasing base strength.1. CH3NH22. NH33. NH2Br4. (CH3)2NH
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic...
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic RNA usually undergoes nuclear processing C. Polypeptides are synthesized by addition of amino acids to the amino terminus. D. The 3' end of mRNA corresponds to the carboxyl terminus of the protein. Grade 2. Which of the following A. It may be autocatalytic. B. Spliceosomes are present in organelles and nuclei C. It involves removal of exons is true regarding RNA processing? D. It...
Part A Arrange the following oxoacids in order of decreasing acid strength. Rank from strongest to...
Part A Arrange the following oxoacids in order of decreasing acid strength. Rank from strongest to weakest acid. To rank items as equivalent, overlap them. 1. HClO2 2. HClO3 3. HBrO 4. HClO Part B Arrange the following carboxylic acids in order of decreasing acid strength. 1. CHCl2COOH 2. CH2ClCOOH 3. CH3COOH 4. CH3CH2COOH Part C Arrange the following amines in order of decreasing base strength. 1. CH3NH2 2. NH3 3. NH2Br 4. (CH3)2NH
6:35 5 minutes ago 25) Which of the following turns off transcription by binding to the operator? A) repressons B) lactose C) RNA polymerase D) promoters E) enzymes 25) 26) In bacteria, what n...
6:35 5 minutes ago 25) Which of the following turns off transcription by binding to the operator? A) repressons B) lactose C) RNA polymerase D) promoters E) enzymes 25) 26) In bacteria, what name is given to a cluster of genes with related functions, along with their control 26) A) exon B) operon C) promoter D) activator E) regulatory gene A mutant bacterial cell has a defective aminoacyl synthetase that attaches a lysine to tRNAs with the anticodon AAA instead...
Part
A
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to the promoter of a gene so that transcription can be initiated.
The closeup shows the secondary structure of the sigma (?)
subunit, which consists of four domains. Identify the domains
labeled 1-3.
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to...
4. A promoter for an E. coli gene that is transcribed by a s-70 RNA polymerase has the following sequence: 30 -20 10 +1 5'GGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA 3'CCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCT The transcription start site +1) is identified. a. Identify the -10 and -35 sequences. How close are they to the consensus-10 and-35 sequences? b. What is the spacing between the -10 and the -35 sequences? How does this compare with the consensus spacing? C. The sequence of bases in a transcribed RNA is identical...
4. Answer the following concerning promoters and transcription. (15 points) a. What role do promoters play in transcription? b. What is the common structure of a bacterial promoter with respect to its consensus sequences? c. What is the role of the sigma subunit for bacterial RNA polymerase as it relates to promoters? d. Eukaryotic promoters are more variable than prokaryotic promoters. Explain why e. Explain what would happen to a eukaryotic promoter if it were associated with acetylated
Below is a diagram of a transcription unit and the mRNA made from the transcription unit: А H DNA TTGACA TATAAT -35 B -10 C D Start codon Stop codon mRNA 5 E F G Is this a prokaryotic or eukaryotic transcription unit? What is the name of the cofactor required for RNA polymerase to bind to the promoter? Which letters on the above diagram correspond to the following structures? Promoters Pribnow box Non-template strand Transcriptional start site- Open Reading...
2. The sequences of 13 promoters recognized by σ70 factor of RNA
polymerase have been aligned below. Deduce the consensus sequences
for the -10 and -35 regions of these promoters. The TSS is
highlighted. Do your consensus sequences agree with the actual
consensus sequences? If not, why?
Gene 1: TCTCAACGTAACACTTTACAGCGGCG--CGTCATTTGATATGATGC-GCCCCGCTTCCCGATAAGGG Gene 2: GATCAAAAAAATACTTGTGCAAAAAA--TTGGGATCCCTATAATGCGCCTCCGTTGAGACGACAACG Gene 3: ATGCATTTTTCCGCTTGTCTTCCTGA--GCCGACTCCCTATAATGCGCCTCCATCGACACGGCGGAT Gene 4: CCTCAAATTCAGGGTTGACTCTGAAA--GAGGAAAGCGTAATATAC-GCCACCTCGCGACAGTGAGC Gene 5: CTGCAATTTTTCTATTGCGGCCTGCG--GAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGAT Gene 6: TTTTAAATTTCCTCTTGTCAGGCCGG--AATAACTCCCTATAATGCGCCACCACTGACACGGAACAA Gene 7: GCAAAAATAAATGCTTGACTCTGTAG--CGGGAAGGCGTATTATGC-ACACCCCGCGCCGCTGAGAA Gene 8: TAACACCGTGCGTGTTGACTATTTTA-CCTCTGGCGGTGATAATGG--TTGCATGTACTAAGGAGGT Gene 9: TATCTCTGGCGGTGTTGACATAAATA-CCACTGGCGGTGATACTGA--GCACATCAGCAGGACGCAC Gene 10: GTGAAACAAAACGGTTGACAACATGA-AGTAAACACGGTACGATGT-ACCACATGAAACGACAGTGA Gene 11: TATCAAAAAGAGTATTGACTTAAAGT-CTAACCTATAGGATACTTA-CAGCCATCGAGAGGGACACG...
Which of the following statements is true? A. RNA polymerase has a proofreading activity B. Prokaryotic RNA usually undergoes nuclear processing C. Polypeptides are synthesized by addition of amino acids to the amino terminus. D. The 3' end of mRNA corresponds to the carboxyl terminus of the protein. Grade 2. Which of the following A. It may be autocatalytic. B. Spliceosomes are present in organelles and nuclei C. It involves removal of exons is true regarding RNA processing? D. It...
6:35 5 minutes ago 25) Which of the following turns off transcription by binding to the operator? A) repressons B) lactose C) RNA polymerase D) promoters E) enzymes 25) 26) In bacteria, what name is given to a cluster of genes with related functions, along with their control 26) A) exon B) operon C) promoter D) activator E) regulatory gene A mutant bacterial cell has a defective aminoacyl synthetase that attaches a lysine to tRNAs with the anticodon AAA instead...
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