Which of the following is an application of immunofluorescence microscopy? Visualization of the surface structures of...
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Explanation- the protein is tagged with a fluorescent tag and the fluorescence emitted by the protein is then detected by the fluorescence microscope.
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Which of the following is an application of immunofluorescence
microscopy?
Visualization of the surface structures of...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
1) Discuss the importance of magnification and resolution in microscopy. How are the magnification and resolution...
1) Discuss the importance of magnification and resolution in microscopy. How are the magnification and resolution of a light microscope different from that of an electron microscope? 2) Which microscope would you use to study the following? a) the changes in shape of a living human white blood cell b) the finest details of the surface texture of a human hair c) the detailed structure of an organelle in a liver cell 3) State the cell theory?...
1. Nucleus * The point from which an activity or process is directed, or on which...
1. Nucleus * The point from which an activity or process is directed, or on which it is focused. Contains genetic information (DNA), controls the cell through production of mRNA and tRNA. The central and most important part of an object, movement, or group, forming the basis for its activity and growth. 2. Mitochondria * This is where aerobic respiration takes place to produce ATP as a form of energy the cell can use. An organelle found in large numbers...
100X The following structure are found in both rokaryotic and cukaryotic cells except (a) mitochondrion (c)...
100X The following structure are found in both rokaryotic and cukaryotic cells except (a) mitochondrion (c) cytosol (b) ribosome (d) plasma membrane What is the maximum total magnification of light microscopy? (a) 5X (c) 100X (b) 40X (d) 1000X 3. The microscopy that scans the specimen with a focused laser beam to obtain a series of two dimensional optical sections, which can be used to reconstruct an image of the specimen in three dimensions (a) SEM (c) TEM (b) Confocal...
(2%) Indicate which secondary structure or structures (α -helix, β -pleated, random coil) will the following...
(2%) Indicate which secondary structure or structures (α -helix,
β -pleated, random coil) will the following peptide adopt in an
aqueous solution at pH 7
(2%) The unfolding of the alpha helix of a polypeptide to a
randomly coiled conformation is accompanied by a large decrease in
a property called specific rotation, a measure of
a solution’s capacity to rotate circularly polarized light.
Polyglutamate, a polypeptide made up of only L-Glu residues, has
the alpha helical
conformation at pH 3....
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
Summarize the following info. Write them in your own words. Make it clear so I can...
Summarize the following info. Write them in your own words. Make it clear so I can read it. Break them into different key points. 5. Significance and Use 5.1 A need exists for accurate data on heat transfer through insulated structures at representative test conditions. The data are needed to judge compliance with specifications and regulations, for design guidance, for research evaluations of the effect of changes in materials or constructions, and for verifi- cation of, or use in, simulation...
Cardiovascular System: page 178-181 Ex-1: Anatomy of Fig. 29.1 and 29.2 Ex-2: Which ventricle is more muscular? Ex-3: Observe tissue slides for human blood smear, artery, and vein;...
Cardiovascular System: page 178-181 Ex-1: Anatomy of Fig. 29.1 and 29.2 Ex-2: Which ventricle is more muscular? Ex-3: Observe tissue slides for human blood smear, artery, and vein; then sketch human blood (based on page 155; Fig. 26.2), artery, and vein Why is this appropriate? Respiratory system: page 182 The respiratory system contains the lungs and those structures that conduct air to and from the lings. The lungs contain brings oxygen into the blood and takes out of the blood...
summatize the following info and break them into differeng key points. write them in yojr own...
summatize the following info and break them into differeng key points. write them in yojr own words
apartus
6.1 Introduction—The design of a successful hot box appa- ratus is influenced by many factors. Before beginning the design of an apparatus meeting this standard, the designer shall review the discussion on the limitations and accuracy, Section 13, discussions of the energy flows in a hot box, Annex A2, the metering box wall loss flow, Annex A3, and flanking loss, Annex...
summarizr the followung info and write them in your own words and break them into different...
summarizr the followung info and write them in your own words and break them into different key points. 6.5 Metering Chamber: 6.5.1 The minimum size of the metering box is governed by the metering area required to obtain a representative test area for the specimen (see 7.2) and for maintenance of reasonable test accuracy. For example, for specimens incorporating air spaces or stud spaces, the metering area shall span an integral number of spaces (see 5.5). The depth of...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
1. Nucleus * The point from which an activity or process is directed, or on which it is focused. Contains genetic information (DNA), controls the cell through production of mRNA and tRNA. The central and most important part of an object, movement, or group, forming the basis for its activity and growth. 2. Mitochondria * This is where aerobic respiration takes place to produce ATP as a form of energy the cell can use. An organelle found in large numbers...
100X The following structure are found in both rokaryotic and cukaryotic cells except (a) mitochondrion (c) cytosol (b) ribosome (d) plasma membrane What is the maximum total magnification of light microscopy? (a) 5X (c) 100X (b) 40X (d) 1000X 3. The microscopy that scans the specimen with a focused laser beam to obtain a series of two dimensional optical sections, which can be used to reconstruct an image of the specimen in three dimensions (a) SEM (c) TEM (b) Confocal...
(2%) Indicate which secondary structure or structures (α -helix,
β -pleated, random coil) will the following peptide adopt in an
aqueous solution at pH 7
(2%) The unfolding of the alpha helix of a polypeptide to a
randomly coiled conformation is accompanied by a large decrease in
a property called specific rotation, a measure of
a solution’s capacity to rotate circularly polarized light.
Polyglutamate, a polypeptide made up of only L-Glu residues, has
the alpha helical
conformation at pH 3....
Cardiovascular System: page 178-181 Ex-1: Anatomy of Fig. 29.1 and 29.2 Ex-2: Which ventricle is more muscular? Ex-3: Observe tissue slides for human blood smear, artery, and vein; then sketch human blood (based on page 155; Fig. 26.2), artery, and vein Why is this appropriate? Respiratory system: page 182 The respiratory system contains the lungs and those structures that conduct air to and from the lings. The lungs contain brings oxygen into the blood and takes out of the blood...
summatize the following info and break them into differeng key points. write them in yojr own words
apartus
6.1 Introduction—The design of a successful hot box appa- ratus is influenced by many factors. Before beginning the design of an apparatus meeting this standard, the designer shall review the discussion on the limitations and accuracy, Section 13, discussions of the energy flows in a hot box, Annex A2, the metering box wall loss flow, Annex A3, and flanking loss, Annex...
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