To place the E. coli replication system on the human scale, imagine that the DNA strand type B has a diameter of 20 angstroms and that it expands to 1m in diameter. If this expansion happened for the holoenzyme DNA polymerase III, it would be about the size of almost half a truck. In this expanded system. (a) How fast each replisome would move. (b) How far would each replisome travel? (c) What size would an Okasaki fragment be? (d) What would be the average distance that the replisome would travel between each error it made? Explain the reasoning and justify your answers, the values must be expressed in km / hr and km.
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To place the E. coli replication system on the human scale, imagine that the DNA strand...
Which aspect of the E. coli DNA replication machinery accounts for high processivity? a. The beta sliding clamp of DNA Polymerase III b. 3’-5’ exonuclease activity of DNA Polymerase I c. 5’-3’ exonuclease activity of DNA Polymerase I d. Single strand DNA binding protein (SSB) e. The core subunit of DNA Polymerase III An explanation of the solution would be greatly appreciated, thanks!
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
describe how the Methyl-directed mismatch repair system in E. coli which DNA strand is the correct strand and determines which DNA strand has the mutation. describe how DNA methylation is heritable during replication how epigenetic modifications are involved in genomic imprinting, X-inactivation, and regulation of tissue/cell specific gene expression (including the general roles of TrxG and PcG group proteins). ***Are these heritable during mitosis or meiosis? Are these reversible? Can you support your answer?