how do you make 500mL of TE buffer (20mM Tris-HCl, 0.75mM EDTA)

Question

Question

Answer 1

Answer #1

Add 10 mL of 1M Tris-HCl + 375 uL (0.375 mL) of 1M EDTA

And make up to 500 mL using water (489.6 mL)

or

Dissolve 1.57594 g of Tris-HCl and 139.59 mg (0.13959 g) of EDTA in 500 mL of water

Note: Molecular weight of Tris-HCl is 157.594

Molecular weight of EDTA is 372.24

Answer 2

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