A 25 yr old female suspected with possible SLE was having an RIA test procedure run. In this procedure, a reduction in radioactivity in the test specimen compared to the radioactive counts measured in the control test is used to quanitate the amount of the patient antibody bound to the antigen with what substance?
Radio ImmunoAssay is a highly sensitive test to measure the concentration of antigen in the patient's sample. In this, a quantity of antigen is reduced with radioactive isotopes of iodine 121 or iodine 135. Then it mixed with the antibody. The sample is then added and the amount of antibody bound with labeled antigen. The antigen and antibody are separated out by using the substance of ammonium sulfate. This also known as farr technique method. This method is used for diagnoses of systemic lupus erythematosus that have high avidity anti-double-stranded DNA.
A 25 yr old female suspected with possible SLE was having an RIA test procedure run....