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Micro Lab: Enterbacteriacease

Yersinia pestis ! Name the sugars in a TSI slant. 20. 21. TSI slants are incubated with the caps loose/tight. 22. Most bacter
what three tests can be determined from SIM media? List an organism that will give a positive and negative results for each t
Indole- Onpg-- Citrate- Urea- H2S-- Fermentation of a carbohydrate-- VP- Lys, orn, arg- Nitrate- _is a mucoid bacterium.
IMVIC Indole MR VP Citrate + + Organism Escherichia coli Edwardsiella tarda Morganella morganii Klebsiella pneumoniae Enterob

Lab 7; Enterobacteriacease Question TSI/SIM
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Answer #1

ANS. 20. Lactose, sucrose, and glucose.

ANS. 21. Loosely for aeration

ANS. 22. Yersinia - 22 to 29-degree celsius

Campylobacter - 30 to 45-degree celsius.

ANS. 25.

alpha-naphthol and potassium hydroxide – VP

Ferric Chloride – TDA

p-Dimethylaminobenzaldehyde – INDOLE

sulfanilic acid – NITRATE

ANS. 26. IMViC : Indole, Methyl red, Voges-Proskauer, Citrate + and H2S.

ANS. 27. Hektoen Enteric Agar

ANS. 28.

INDOLE - blue to blue-green

ONPG - yellow

CITRATE - blue

UREA - orange

H2S - black

FERMENTATION OF CARBOHYDRATE - orange to yellow

VP - pink-red

NITRATE - cherry red

ANS. 29. Pseudomonas aeruginosa is a mucoid bacterium.

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