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4. (2pts) Make sure you explain your answers. N=2 000 plates N = 7 500 plates etector response A) Which chromatogram represen
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A) Chromatogram b show better resolution that that of chromatogram a . because Peak width of the chromatogram (a) is greater that that of chromatogram (b ) as separation is not efficient of chromatogram (a) peak become broad.

Peaks from chromatogram (a) are sharp and peak width also less .

B) As particle size decrease the diffusion path lenghts are short allow to solute in and out faster .and peak dispersion become less .

Partical size of column from which chromatogram (b) is run is smaller than the other chromatogram.

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