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Answer 1

Why is new methylene blue preffered every new cresyl blue.

New methylene blue (also NMB) is an organic compound of the thiazine class of heterocycles. It is used as a stain and as an antimicrobial agent. It is classified as an azine dye, and the chromophore is a cation, the anion is often unspecified.

The Brilliant cresyl blue solution is used in hematology as reagent for the staining of blood for the subsequent manual counting of blood cells.  The regenerative limit of erythrocytes can be observed with the proportion of reticulocytes. Moreover, the assurance of the Substantia granulo-filamentosa (ribonucleoproteins) is conceivable with new, unfixed youthful erythrocytes (in a supra-vitral recoloring). The Brilliant cresyl blue arrangement is an IVD and CE item and 100 ml of the arrangement are equipped for recoloring up to 5000 slides.

The ultrastructure of redox-dye-treated HbH-containing red cells has been shown to be dependent on the nature of the redox dye used. Brilliant cresyl blue causes the formation of a large number of small inclusions which are invariably attached to the inner surface of the red cell membrane. Methylene blue and new methylene blue reason the development of an a lot more modest number of bigger considerations just some of which give off an impression of being film bound. These ultrastructural contrasts are reflected in checked contrasts in the light magnifying instrument appearances of the supravitally-recolored cells.