Question

What happened to the spinach protein?

Which layer would the proteins be in?

Background needed:

We ground up a spinach leaf in a mortar for 5 minutes with 3 mL of acetone and 3 mL of hexane. We then filtered the green solution (avoiding the ground leaves) into a filtering pipet. 2 mL of saturated aqueous sodium chloride were added to the test tube with the filtered solution in order to make **two layers**. The top, more green layer was then transferred through a filter pipet with solid sodium sulfate. The solution that was filtered was then put in a 70 degree Celsius hot water bath until the solvent had evaporated to about 0.2 to 0.3 mL. After all this, the solution was put through TLC.

Answer 1

On heating the filtered layer to dryness in 70 degrees the spincah protein will get denatured.

Since proteins consists of amino acid group and carboxylic acid group, it will be present in organic layer which is the acetone layer.