Question

Normal 50% COHD Volume percent oxygen 10 ml/100 ml blood) 50% anemia 0 100 20 40 60 80 Pressure O, (mmHg) a. (5 points) Sketch oxygen binding curve for Myoglobin on the diagram given above. b. (5 points) Explain what is Bohr effect and sketch oxygen binding curve that displays Bohr effect in the above diagram. c. (5 points) Dotted curve corresponds to hemoglobin that is exposed to carbon monoxide. Account for the shift of the curve and the shape of the curve? d. (5 points) Dashed curve corresponds to hemoglobin binding. Account for the shift of the curve and the shape of the curve? e. Extra credit (5 points) Imagine you are working on a research project to construct a hemoglobin variant that has higher affinity for oxygen under physiological conditions by site directed mutagenesis. (Replacing original amino acids by other amino acids)

Answer 1

as Oxygen binding curtre for Myoglobin. Saturatim saturation/volm I Oz partial pressure 5) Bohr effect: Bodir effect is an effect that is observed when increase in co₂ in blood stream or decreasing pH in arterial environment results in unloading more amount of ₂ from Hb. It helps physiologically to obtain more O2 from blood stream during excess CO₂ consumption %0₂ satoration ---low pH, High CO2 ... Normal pH, normal co₂ 1-. -- High pH, low co, Y. O2 pressure e) co produces a contain tissue by impairing Oxygen transport and interfereing with eytochrome onedase fonction . Absorbed rapidly via the lungs, co binds avidly to tb forming QHb whose affinity is 200 times more than ony Hb. This tight binding of Oz to Hb resulting in a leftward shift of the onggen-te dissociation curve, giving a satorated conce at low Oat. d) Then anemia develops. The ony hemoglobin dissociation corre is shifted to the right. Ho has a decreased affinity ton towards O2 dand releases on at a higher partial pressure.