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Given the following data (generated from a series of assays), what is the Km and Vmax...

Given the following data (generated from a series of assays), what is the Km and Vmax for this enzyme? Include units and be careful of significant figures.

[S], μM V(μM/min)
10.0 32.4
25.0 66.3
50.0 102
150.0 160
300.0 185
0 0
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Answer #1

V(uM/min) (43.6, 92.5) TIITTO 50 100 200 250 300 150 S(UM)

Here, V max is 185micro,M/min and Km is that value of x axis which corresponds to the value of Vmax/2 in the y axis. Here, Km is 43.6microM.

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