Homework Answers
Ex 5.5 catalase test
- Aerobic bacteria requires presence of oxygen to carry out respiration. Facultative anaerobes can carry out respiration in presence of as well as absence of oxygen.
The enzyme produced by aerobic and facultative bacteria are superoxide dismutase and catalase.
- During aerobic respiration, hydrogen peroxide is formed as the byproduct. Hydrogen peroxide is a reactive oxygen species and damage the cell. Catalase helps decompose the hydrogen peroxide to harmless products oxygen and water.
- The reagent used for checking catalase is hydrogen peroxide.
- When hydrogen peroxide is added to catalase, catalase causes enzymatic decomposition of hydrogen peroxide to oxygen and water. Production of oxygen results in bubble formation.
Ex 5.6 oxidase test
- Oxidase test is used to determine the presence of enzyme cytochrome oxidase. Cytochrome oxidase is produced only in aerobes and thus its presence determines whether the bacteria is aerobic or anaerobic.
- Oxidase enzymes participates in oxidation reduction reaction in electron transport chain. It receives an electron and transport it to dioxygen molecule in electron transport chain. This causes conversion of dioxygen to molecular oxygen and water resulting in synthesis of ATP.
- The test used for oxidase is called cytochrome oxidase test.
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UNTUWI Pre-lab Respiration Tests Ex 5-5: Catalase Test Pre-lab questions: What are the two enzymes produced...
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catalase test
EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart. Bacteria Foaming or No Foaming Catalase Present Absent M. luteus S. aureus S. lactis 1. Review Questions: 1. Explain why the enzyme catalase is essential for the survival of many aerobic and facultative anaerobic organisms? 2. Explain how catalase negative bacteria are capable of growing in the presence of oxygen Experiment 30 Catalase Test 273 3. Can bacterial cultures grown on blood agar be...
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used...
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used to test for Oxidase? 5. Which families of organisms are differentiated by the Catalase test? What is a positive test for catalase? 6. What substrate is used to test for the presence of the Coagulase enzyme? What does Coagulase do for a bacteria that has it?
Experiment 9: Pre-Lab Questions 1. Why is respiration important for living organisms? 2. (Circle one) a....
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help please! 81% Help % Lab 39 worksheet 25% + Room Add Page Insert Durham Tube...
help please!
81% Help % Lab 39 worksheet 25% + Room Add Page Insert Durham Tube Sugar Fermentations Table Chart Text Shape Media Comment 1. Which tube, red or yellow, is positive for sugar fermentation? 2. Is the color change an increase or decrease in pH? 3. Which two gases can be revealed by the bubble in the inverted tube? Mixed Acid (MR test) and Butanediol production (Voges:Proskauer, VP test) 1. Which group of bacteria is the Mixed Acid test...
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37. What does the enzyme catalase do (also see Chapter 6)? a. Detoxifies H.O, created in...
37. What does the enzyme catalase do (also see Chapter 6)? a. Detoxifies H.O, created in the oxidation of flavoprotein in the e' transport chain b. Detoxifies H,O, created by the action of superoxide dismutase c. Allows many aerobic, facultatively anaerobic, and microaerophil microbes to survive d. All of the above 38. What type of media is used in the catalase test (we will use the test tube version, not the slide) a. Agar plate b. Agar slant c. Agar...
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Which genera of Bacteria can be diffeh Morganella, Proteus, and Providencia EX 5-20: SIM Medium Pre lab Questions List the three bacterial activities that can be tested using SIM medium? -Sulur reduction - indole production fron tryptophan motility Which amino ácid in the medium is key to test sulfur reduction using this medium? What is the product of sulfur reduction? How does the black color, indicative of sulfur reduction, develop in the medium? any blaukening of the medium is an...
catalase test
EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart. Bacteria Foaming or No Foaming Catalase Present Absent M. luteus S. aureus S. lactis 1. Review Questions: 1. Explain why the enzyme catalase is essential for the survival of many aerobic and facultative anaerobic organisms? 2. Explain how catalase negative bacteria are capable of growing in the presence of oxygen Experiment 30 Catalase Test 273 3. Can bacterial cultures grown on blood agar be...
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used to test for Oxidase? 5. Which families of organisms are differentiated by the Catalase test? What is a positive test for catalase? 6. What substrate is used to test for the presence of the Coagulase enzyme? What does Coagulase do for a bacteria that has it?
Experiment 9: Pre-Lab Questions 1. Why is respiration important for living organisms? 2. (Circle one) a. Yeast is heterotrophic or autotrophic b. TRUE OR FALSE. Yeast can carry out both aerobic and anaerobic cellular respiration. 3. What gas will be produced by the yeast, making the balloon bigger? 4. Explain the difference between aerobic respiration and anaerobic respiration. Which is more efficient in terms of energy? 5. What is the purpose of using various temperatures in this experiment? Explain 116
help please!
81% Help % Lab 39 worksheet 25% + Room Add Page Insert Durham Tube Sugar Fermentations Table Chart Text Shape Media Comment 1. Which tube, red or yellow, is positive for sugar fermentation? 2. Is the color change an increase or decrease in pH? 3. Which two gases can be revealed by the bubble in the inverted tube? Mixed Acid (MR test) and Butanediol production (Voges:Proskauer, VP test) 1. Which group of bacteria is the Mixed Acid test...
Lab Report: Ex 2-1: Ubiquity of Microorganisms Pre-lab Questions Purpose: What is the purpose of this Lab? What is the major difference between free-living organisms and pathogens? What are opportunistic pathogens?
37. What does the enzyme catalase do (also see Chapter 6)? a. Detoxifies H.O, created in the oxidation of flavoprotein in the e' transport chain b. Detoxifies H,O, created by the action of superoxide dismutase c. Allows many aerobic, facultatively anaerobic, and microaerophil microbes to survive d. All of the above 38. What type of media is used in the catalase test (we will use the test tube version, not the slide) a. Agar plate b. Agar slant c. Agar...
mlology of Gastrointestinal illness: Differentiation of Enterobacteriaceae 345 com a mixed ive and dif- PRE-LAB QUESTIONS Remember, Understand, Apply is 1. Resident enteric bacteria make it hard for pathogenic organisms to colonize the gut because the large resident population usually out-competes the pathogenic organisms for nutrients. a. true b. false 2. Facultative anaerobes are able to survive outside the large intestine. a. true b. false 3. The microbial population found in the large intestine , which makes the isolation of...
Pre-Lab Study Questions 16 How can the presence of an ion in a solution be detected 2. What tests would you use to identify a solution of Ag3PO4? 3. What tests would you use to identify a solution of FeCly? 4. A flame test of a colorless solution gives a bright yellow color. When reacted with AgNO, a white precipitate forms that dissolves when HNO3 is added. When HCl is added to the unknown solution, bubbles form. What is the...
6. What is the function of the enzyme urease? 7. What is the purpose of including phenol red (PR) in the urea broth? What compound is responsible for the rise in pH? edients . فمفد 8. What happens to the gelatin inoculated with a gelatinase-positive organism? 9. What reagent is used to test for the presence of indole? What happens to the reagent layer if the indole test is positive? 10. What does SIM medium test for? m SECTION 5...
Which genera of Bacteria can be diffeh Morganella, Proteus, and Providencia EX 5-20: SIM Medium Pre lab Questions List the three bacterial activities that can be tested using SIM medium? -Sulur reduction - indole production fron tryptophan motility Which amino ácid in the medium is key to test sulfur reduction using this medium? What is the product of sulfur reduction? How does the black color, indicative of sulfur reduction, develop in the medium? any blaukening of the medium is an...
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