Question

Picarate dehydrogenase (PDH) catalyzes the reduction of dextoacetate to picarate, while oxidizing NADH to NAD⁺. The overall reaction and the active site of PDH is shown below. Use curved arrow notation to propose a reasonable reaction mechanism for this reaction.

НО, o НО 0 + NADH + H+ LOH + NAD+ picarate dehydrogenase 6/ 0 dextoacetate picarate

Answer 1

Reaction mechanism : Hozo H + HO = TI pa oe o a Note: here first oxygen lone pair react with Htions further NADH donates Hion to reduce from keto group to secondary alcohol, here NADH oxidation tackes place it get converts NAD HO attacke tankes placé NOAH J NADO HOVO LO-H product