Question
a) which amino acid tested is most polar?
b) look up the structure of the amino acids. explain why it makes sense that the amino acid you listed is more polar than the others?
c) what amino acids are in the fruit juice?
D. CHROMATOGRAPHY Chromatography is a general technique that is used to separate various materials. In this case, students ca
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Answer #1

a) which amino acid tested is most polar?

Answer: Glutamic acid

b) look up the structure of the amino acids. explain why it makes sense that the amino acid you listed is more polar than the others?

Answer: It has two polar electrically charged carboxylate anions, overall charge = -1, whereas Serine has only one carboxylate anion and is electrically neutral, in Isoleucine hydrocarbon chain makes it non-polar and electrically neutral.

HjC-CH CH2 CH3 Isoleucine (lle) H3N-- Electrically POLAR NONPOLAR Charged Serine (Ser) Hync- CH2 Chy Acidic Glutamic Acid (Gl

c) what amino acids are in the fruit juice?

Answer: From TLC it is clear that the fruit juice contains glutamic acid as its Rf value exactly matches with that.

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