Question

3. Indicate the (major) class of enzym major class of enzyme that would be required to catalyze each of the following reactions. The options include: oxidoreductase, isomerase, transferase, hydrolase, lyase, and ligase. . - 4. Consider the following 31 residue polypeptide N-Terminal 9000 C-Terminal a. Predict how the following denaturants will affect the folded structure of this polypeptide. What specific regions (or attractive forces/covalent linkages) will be affected by each of these reagents? Use the residue numbers to locate particular regions of the protein. i. High concentration of NaCl(aq) ii. Mercaptoethanol (HOCHCHSH) iii. Sodium dodecylsulfate (a detergent)

Answer 1

Answer 3a)

The reaction involves oxidation of substrate from C-OH to C=O.

So, enzyme is oxidoreductase.

Answer 3b)

The reaction involves transfer of methyl group from reactant 2 to reactant 1.

So, enzyme is transferase.

Answer 4

a) High concentration of NaCl will affect the electrostatic interaction between Asp and Arg.

Na⁺ and Cl^- ions will interact with ionic forms of Asp and Arg and will disrupt their electrostatic interaction.

Residues affected will be 15 and 18.

b) Mercaptoethanol will denature protein by disrupting disulfide bonds.

Residues affected will be 2 and 6

c) Sodium dodecylsulfate

It denatures protein by interacting with hydrophobic part of each residue in protein chain and then imparts negative charge to the protein chain.

All residues will be affected.