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You have a solution of sucrose, and a few mL of 0.4M HCl is added to...

You have a solution of sucrose, and a few mL of 0.4M HCl is added to the solution. The solution is heated.

What reaction will you see when adding Benedict's reagent?

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Answer #1

Sucrose contains two reducing sugars molecules fructose and glucose. These sugar are joined by together by glycosidic bond prevent the glucose isomerising to aldehyde, and fructose to alpha-hydroxy-ketone. Thus, Sucrose is a non-reducing sugar which do not show positive Benedict’s reagent.

But as you heat the Sucrose with dilute hydrochloric acid(HCl) molecule is broken into two molecules (reducing sugars). The acidic conditions, heat break the glycosidic bond. The products of hydrolysis process are glucose and fructose which can be detected by Benedict’s reagent.

glucose/fructose + Cu(citrate)22- (benedict reagent) -----------------> carboxylate anion + Cu2O (brick red ppt)

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