Question

2) A blotting method known as Western blotting can be used to detect proteins that are translated from a particular mRNA This techniques first separates proteins by size using electrophoresis then detects specific proteins through staining (antibody directed). Key is that you can visualize only the protein of interest on the gel. Suppose a researcher was interested in the effects of mutations on the expression of a gene that encodes protein X This protein is expressed in the skin and contains 654 amino acids with a molecular mass of 80 kDa Make a drawing that shows the expected results of a Western blot for this protein using skin cells that were obtained from the following individuals. Box indicates the well. Indicate lane (top) and sizes (left side) of the image. Include sizes on your image. Lane 1: Individual who is homozygous for a mutation within the enhancer region blocking transcription factor binding Lane 2: Individual who is heterozygous in which one gene is normal and the other has a mutation that introduces an early stop codon at codon 521 Lane 3: Normal Individual Lane 4. Individual who is homozy gous for a mutation that introduces an early stop codon at codon 225 Lane S: Indirvidual who is heterozygous for a mutation that changes codon 198 from a Lysine Lane 6: Indvidual yho is homozy gous for a deletion which removes the promoter for this gene codon into a Phenylalanime codon, whiie other gene is normal

Answer 1

Individual who is homozygous for a mutation that introduce an early stop an codon at codon 225.