1) If you have a 5 molar solution and a diluted 0.1 molar solution. What is the ratio between these two?
2) If you want to prepare 50ml medium containing 5% FBS. What is the volume of FBS you have to add?
3) If the concentration in your original medium is 50.000 cells per ml and you add 1ml of this medium to 4ml fresh medium without cells. How many cells per ml are in these 5ml of new medium?
-The original medium has been diluted ..... fold.
-This means it has been diluted in a ratio of ........
The answers are based on understanding of simple mathematics and mostly based on unitary method.
1) If you have a 5 molar solution and a diluted 0.1 molar solution. What is...
Fill in the Blanks. - A 1:10 ration, means that the final solution has been diluted ....... fold. - If the original solution has 0.1 mole per liter and the ration is 1:5, the final concentration is ........ moles per liter. - You have a 1 molar solution and need to do a 1:5 dilution to prepare a 40ml solution. This means you need ....... ml of the original 1 molar solution for this dilution.
You have a bacterial culture solution. When you directly measured the OD600, of the culture solution, it was 3. Then, you performed a 10-fold dilution and measured the OD600 of the diluted solution, which was 0.5. Thirdly, you performed another 10-fold dilution and measured the OD600 of the diluted solution, which was 0.07. Assuming that OD 600 =1 is equivalent to 10^9 cells/ml, how many cells do you have in 1ml of the original culture? You have a bacterial culture...
You have a 5 mg/ml solution of caffeine (molar mass 194.2 g/mol). You diluted that solution 200 times (200 fold),. Calculate the final concentration (in uM, micromol/t) of caffeine after dilution 12.9 uM 19.4 uM 129 uM 194.2 uM
9. A. You have diluted a sample by 1000 fold and plated 1 mL on an agar plate. You observe 35 colonies. What was the concentration of the original sample in CFU/mL?
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
You have titrated 25.0 mL of acid A with 65.35 mL of a 0.231 molar solution of base B. The mole ratio is 1 A = 1 B. What is the concentration of the solution of A?The answer is 0.604,but i don't know the process
1. You have a 1L solution of 0.1 M phosphate buffer at pH 2.0. What is the pH of this solution after you add 5 grams of NaOH? Assume there is no volume change and phosphate buffer has the following pKa values: 2.15, 6.82 and 12.38.
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
You have a purified alcohol dehydrogenase from liver. You take 50 micro liters of this solution, and add 950 micro liters of buffer. The absorbance of the diluted solution is 0.542. A) what is the ADH concentration in the undiluted sample (in mg/ml)? The extinction coefficient for a 0.1% solution of ADH at 280 Bm is 1.83. B) what is the ADH concentration in micro M? (The molecular weight of ADH is 140,000 Da)
1. Why do you dilute the fibroblasts in trypan blue before counting them? What will the trypan blue tell you about the cells? 2. Why do you add trypsin to the fibroblasts in the culture dish? How does trypsin do this? 3. You have counted the 4 corner squares of a hemocytometer grid (each square is 1mm2 and the distance of the coverslip above the squares is 0.1mm) and have come up with a total cell count of 243 cells. What is your original...