Question

traditional NCLEX question format. A triage nurse in the ER begins the assessment of an elderly patient complaining of bloody diarrhea and severe stomach cramps. The patient has a fever of 1040F as well, and is showing signs of dehydration. IV fluids are administered while further workup of the patient continues Testing is performed on the specimen in the laboratory, including the Gram stain. This test separates bacteria into two broad classes: gram- positive and gram-negative. Which step in the staining process is the differential step, for it only acts upon one type of cell? O a) Addition of safranin (counterstain) b) Addition of crystal violet c) Addition of Gram's iodine (mordant) d) Decolorization with alcohol MacB esc 20 F1 F2 F3 F5 4

Answer 1

Correct answer: option b

Rational:

Gram positive organism can retain crystal violet where as gram negative can not retain the crystal violet.

So option b is correct.

Other options are incorrect.