Question

Name Immunology Laboratory Worksheet- Indirect ELISA A patient is suffering from recurrent yeast and bacterial infections. His blood sample is collected for HIV detection. You are a new lab personnel assigned to perform the test. Make a flowchart of the procedure you plan to follow and list all the materials you require for the test. 1.

Answer 1

Q. No. 1. Answer :

Procedure for indirect Elisa test for HIV :

* first we have to collect blood samples from client.

* add antigen to that.

* then wash it.

* add controls and unknown and incubate it.

* then again wash it.

* next add the enzyme linked secondary antibody , then incubate it,

* and wash it,

* then add substrate and incubate,

* finally analyse the results.

Needed articles or equipment or materials for doing test :

* sterile gloves to wear to hands,

* syringes to draw blood samples from client.

* test tubes to collect and store blood.

* tarnquit to tigh to the hand of the client.

* antigen solutions,

* reagents,

* centrifuser,

* plastic containers,

* kidney tray and paper bag to collect waste.