Question

values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret Test Color with Biuret Reagent Test Tube Contents Questions: 1) Which tubes contain protein? Describe a positive biuret test for protein based on your experimental results. the Rpomin Cole tor 丹 ts in proteins 2) Based on your qualitative (visual) observations of the Biuret test results, do all three tubes contain the same amount of protein? How do you know? 3) Explain method by which you could quantify the amount of protein in each tube in table 2. (Hint: remember how you quantified the amount of starch in solution in Experiments 10 and 11?) 162

Answer 1

Table 2

2. Only albumin solution only had presence of protein in biuret test .because remaining things did not have proteins because there is no presence of amino acids or peptide bonds in that solution.

3. Complication of copper ions to functional groups in the proteins peptidbonds.the formation of copper ions protein needs two peptide bonds and produce the violet colour and measured by absorption spectroscopy at 540nm.

Table 3

1. In heat

Changes in the ph level affect the amino acids and lead to denaturation

In acids

Secondary and tertiary structures are altered but the peptide bonds of the primary structure between amino acids are not altered once it is denatured the protein can no longer functioning.

In salt

Increases in sodium chloride present in the egg white solution the salt molecules readily interact with water .egg proteins are calculated and get denatured.

Alcohol

Same as in heat

Heavy metal ions - same as in acids

2.other methods are ,

Electrostatic forces

Hydrogen bonds

Hydrophobic force

Sisulfide bonds and others are temperature , acid, salts and ph level

These are all alter the structures of protein and denatured it.

3 .proteins can be denatured by heat , acid, salt , and gravy metals. The swallowed poison can react with the proteins of milk or egg white for denaturing. Rather than on the protein sites and tissues in the stomach or mouth. It will stimulate the nausea and vomiting. So the poisons will vomiting with egg white or milk while in case of ingestion of heavy metals ions.