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Scenario 4: After speaking with Alexander Pierce, Captain America (Steve Rogers) gets into the elevator to head down to opera

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The first symptom is dialated eyes of agent rumlow.

Our eyes get dialated when we are in adrenalin rush during fight or flight situation.

The second symptom is the people are not able to make eye contact.

When people feel anxious about you knowing about their lies they do not make eye contact.

And thirdly the man are highly sweating even when they entered from air conditioned building.

It is also a fight or flight response as adrenalin activates sweat glands.

2) Sympathetic nervous system is activated in fight or flight response.

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