Question

i just need help with 2 & 3 & 4. please explain and help

8. Rf value of compound on a TLC plate: h. "Like dissolves like" 2. What does one achieve by doing Thin Layer Chromatography or a column chromatography on a mixture of organic compounds? o 3. What is the basis of separation of two or more organic compounds when they are subjected to TLC? ano 4. Unexpected result: Why do you think the elution sequence of the two dyes you found out was reversed in your TLC and Column chromatography experiments? mugavebben

Answer 1

2) TLC and column chromatography

1) TLC can be used to seperate different organic Compound mixtures. TLC is just analytical tool to quickly determine eit míztele iis pure how many compounds it contain, jy vreaction is complete etc ILC is 4oy very very small amount of sample. Column Chromatography is bigger Virsion of TLC. only difference is stationary phase is not applied on plote but packed so in one columo. This Chromatography is used to seperate the mixture and purify the compounds. TLC works on principle of seperation on the basis of affinity of compounds towords both the phouse that is stationary and mobile phase. also polarity is the factor. more polay compound within creen with more polar mobile phase and non polar will not run ou move up slowly.

1) TLC can be used to seperate different organic Compound mixtures. TLC is just analytical tool to quickly determine eit míztele iis pure how many compounds it contain, jy vreaction is complete etc ILC is 4oy very very small amount of sample. Column Chromatography is bigger Virsion of TLC. only difference is stationary phase is not applied on plote but packed so in one columo. This Chromatography is used to seperate the mixture and purify the compounds. TLC works on principle of seperation on the basis of affinity of compounds towords both the phouse that is stationary and mobile phase. also polarity is the factor. more polay compound within creen with more polar mobile phase and non polar will not run ou move up slowly.

4) The only reason which caused eveversed elition sequence of two dyes is change co polarity of mobile phase in & More polar compound runs fast and elicte out winst 'When mobile phase. Les polour Polvert but when non-polar mobile phase is Lesed, then polar compound which ceseal to clete girst will now own slowly and clute out clast.

Answer 2

2.

Thin layer chromatography (TLC) and column chromatography are used to separate mixture to its pure components.   A mixture is composed of several organic compounds. When the mixture is passed through the column or run on a TLC plate, the compounds in the mixture depending on their polarity interact differently with the stationary phase of the TLC plate or the column. Depending on the interactions, different compounds in the mixture move at a different pace and come out of the column or move up the TLC plate.

3.

The basis is the polarity of the different organic compounds in the mixture and the polarity of the stationary phase. For a polar stationary phase (i.e the polar adsorbent material on the plate), the polar compounds move up the plate much slower than the non-polar compounds. The polar compounds move slower because of the dipole-dipole intermolecular interaction between the polar compounds and polar stationary phase. Hence, two or more compounds show different R_f values.