Question

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2 A solution containing 52.4 mg/100ml of B (335 g/mole) has a transmittance of 45.2% in a 1.0 cm cell at 400nm. Calculate the "ABSORPTIVITY" of "B" at this wavelength Inna m L v inole -,.С/.x103 i-moll A

Answer 1

2. The relationship between absorbance (d) and percent transmittance(907) is as follows A 2-log (%T) -2-log(45.2) -0.34 The relationship between absorbance (A )and concentration (c) is as follows Aabc Here, a is the molar ab sorptivity and bis the pathlemngth. 52.4 mg 1 mol 0.00156 mol/L 100 mL 335 g 0.34 2 1 8 L/(mol cm) a=-= = bc 1.0 cm) (0.00156 mol/L) Therefore, the molar absorptivity of B is 218 L/(mol cm).