Question

Ion Exchange Chromatography Experiment
1. What is the basis for the seperation of different compounds by ion exchange?
2. How can molecules with the same charge at varying amounts be seperated by chromatography?
3. Why are celluloses often used as supports to seperate large biologically active proteins?
4. Why is it important to prepare a standard curve for each spectrophotometer?
5. What would happen if 0.5M K+ acetate were used to elute the sample? Why?
(0.01M KOAc was actually used)

Answer 1

Answer 1: Ion exchange chromatography is used to separate the different compounds on the basis of their chage. By using this technique purification of biomolecules was carried out. During this process, thier is a interaction between the charged molecules in given sample with oppositely charged functionalities on the stationary phase.