Question

. You record initial rates for the activity of 5 HM enzyme, E, in the presence of 1.0, 2.0, 5.0, and 10.0 HIM substrate S. Plotting the steady-state activity curve yielded K,mof 9.5 HM and car of 0.17s. However, the literature values for this enzyme are Km of 1.0 HIM and keat of 0.1 s'. What is the most likely reason for your Kar and Keat values not matching those from the literature? a. Experimental error. b. The experiment was not performed in pseudo-first order conditions. c. The enzyme or the substrate has degraded. d. An inhibitor is present in this enzyme preparation.

Answer 1