Question

#4. Now use Beer's Law (shown be above to determine the molar absorp calculations and include the pro A=bC hown below) and 1 concentration/absorbance pair from question #2 olar absorptivity coefficient for Chlorophyll a. Show your Include the proper number of significant digits in your ans C: 22 A-1.1704 1.1704 = å b(.22) #5. Now, calculate the Now, calculate the concentration of a sample of Chlorophyll a with an absorbance of 3.33 using only Beer's Law. Show your calculations and include the proper number of significant digits in your answer!! A-333 333=&bc

Answer 1

4. acc to Beer's Law-

A=$\epsilon$*C*l

A=Absorbance = 0.266

$\epsilon$ = Molar Absorptivity

C= concentration = 0.05M

l =Length of cuvette = 1cm (Standard value considered since not specified)

Now,

0.266 = $\epsilon$ * 0.05M *1cm

$\epsilon$ = 5.32M-1cm-1.

5. If A= 3.33

A=$\epsilon$*C*l

3.33 = 5.32M-1cm-1 * C * 1cm

C = 0.626 M