Question

Analysis of Co?by Spectrophotometry 3. Utilizing the data below to calculate the absorbances of your dilutions from part 2 % Transmittance This exam involves using best practices to analyze a Counknown solution. Multiple working standard solutions will be prepared bydiluting a stock solution of Cocle The 0.200M stock solution will be the highest concentration standard it has been analyzed to give the table (below) of absorbances as a function of wavelength. This analysis will be done by spectrophotometry. Measurements are done in 120cm cuvettes. The stock solution glassware, and the spectrophotometer are provided. Everything else is your responsibility Known Concentration 0.20 0.18 0 13 0.16 0.14 0.12 16 20.1 26 Wavelength Absorbance 0026 0.130 10. Generate a plot of the lombdo vs absorbance. Submit this graph along with the completed word document. 0.414 400 420 440 460 480 500 20 140 0.84 0911 0704 0374 Ib. From the absorption spectrum, calculate the value of the molar absorption coefficient at. Make sure to include units 50 600 620 640 0.104 0078 0.050 2. You are provided 50.0 mL of the 0.200M Col, stock solution. With this stock solution prepare dilutions to produce a series of known concentration solutions. Show all dilution calculations below. Type or drow the equations and solution concentrations.

Answer 1

1 09. 0.8 0.7 0.6 Absorbance 0.5 0.4 0.3 0.2 0.1 0 100 200 300 400 500 600 700 Wavelength

From 11 1 max graph, 5 16 am Imax Molar absorption core Hicient at 0.018 s Absorbance 2 Log Ilog

1.2 1 y = 4.946x -0.000 R? = 0.999 Solution concentration Absorbance 0 0 0.2 0.18 0.886056648 0.16 0.795880017 0.14 0.696803943 0.12 0.585026652 0.8 0.6 Absorbance 0.4 0.2 0 0.05 0.1 0.15 0.2 0.25 -0.2 Concentration