Question

Question 3 As discussed in class, the initial step of Edman degradation involves a reaction between the free amine of a peptide and phenyl isothiocyanate. The process of adding moieties to the N-terminus of a peptide is often called "peptide derivatization," and it has many uses outside of peptide sequencing. One such reaction is shown below. CI Cl H2N N-Peptide C) Peptide CQ C) C) 2 HCI C) Propose a curved-arrow mechanism for this transformation, showing all reaction intermediates and co-products. You may use general acids and bases, if necessary.

Answer 1

NH- P Base exrads HO R. +Co + cl