Question

2. Suppose there is a beaker with some red blood cells (RBC) in a buffer solution. There is a variation of the RBC concentration along the depth direction. Design a RBC sensor which can measure the RBC concentrations at various depths with the following considerations: (1) Detect only the local RBC concentration in situ; (2) minimize the disturbance to the surrounding and (3) Minimize background noise.

(a) Choose an optical detection method (e.g., SPR, fluorescence, FRET, TIR, etc.).

(b) Sketch the detection mechanism.

(c) Describe the operational principle