Question

6. Four common matrix molecules used for MALDI are listed in the table below, along with the typical wavelengths used. Compare and contrast the structures of these molecules (include the structure of each in your answer). What does the structure of the molecules have to do with the differences in the wavelengths used? Compound Nicotinic acid Vanillic Acid 3-amino-pyrazine-2-carboxylic acid Succinic acid Wavelength Range (nm) 220-290 2a 337 2940, 10600

Answer 1

The MALDI technique has revolutionized the analysis of molecular weight non - compounds, especially polymers and his polymers with molecular weight up to 300000 Dolton�s. This technique is completed in two steps. First, a solution of event, analyze, and matrix compound are thoroughly mixed and placed on a disk to dry. As the solvent cry stables of matrix contain every dispersed analyze molecules are portend. In the second step, the coated disk is placed in the vacuum chamber of the MS then the disk is repeatedly pulsed with a leer in the UV or visible spectrum depending on the matrix. During each the matrix molecules are rapidly valorised and carry the analyze molecular into a low pressure plasma the wave length of the is selected to heat and volatize the matrix and to avoid significant that or degrade the analyze molecular Analyze molecules are mostly ionized in the vapor phase of ionization, excited - state proton