Homework Answers
A standard curve is also known as a calibration curve which is used for the quantitative research techniques. It determines the same components present in multiple samples by measurements and plotting graphs.
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1- What is a standard curve and how is it used in biochemistry? 2- Explain how...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a 500.0 ppm stock solution and diluting it to a final volume of 360.0 mL. Working standard 2 is prepared by taking 3.00 mL of working standard 1 and diluting it to a final volume of 250.0 mL. Calculate the ppm concentration of the working standard 2. Provide your answer to three places after the decimal and without units. Your Answer: Answer Question 5 (2...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
Question 1 Referring to Table 1 on page 9, what is the final concentration of Fe+2...
Question 1
Referring to Table 1 on page 9, what is the final concentration
of Fe+2 in ppm of the last standard solution
assuming that you used exactly the target value of 8.00 ml
Fe+2 in the 100 mL flask? For this question, assume that
you added exactly the volumes of all reagents as listed in the
table. (Remember that the stock solution concentration is 50.00
ppm.) Show all your work carefully
using good form and significant figures.
Table 1: Components...
THE DETERMINATION OF IRON BY SPECTROPHOTOMETRY INTRODUCTION In this experiment, the red-orange colored complex formed between...
THE DETERMINATION OF IRON BY SPECTROPHOTOMETRY INTRODUCTION In this experiment, the red-orange colored complex formed between iron(II) and 1,10- phenanthroline (Eqn.) is used in determination of iron by spectrophotometry. Fe+3PhenH Fe(Phen)2 +3H red-orange (A 512 nm) An excess of reducing reagent, such as hydroxylamine or hydroquinone, is often used to reduce and maintain iron in +2 oxidation state. The complex, once formed, is very stable, and can be stored for a long time. Required Reading: Skoog and West (9E): Chapter...
To determine the Ca^2+ concentration in water sample, a standard EDTA solution of 0.01988 M was...
To determine the Ca^2+ concentration in water sample, a standard EDTA solution of 0.01988 M was used to titrate 25 ml. of the sample solution with the presence of an ammonium buffer (pH 10). If 15.80 ml. of the standard EDTA was used to reach the end point, calculate the molar concentration and the ppm concentration of the unknown Ca^2+ (atomic weight of Ca = 40.08g/mole) solution The amount of 0.2915g of benzoic acid was dissolved in 100 ml. of...
DATA SHEET Overall Stoichiometric Equations 1. Results and Observations 2. Unknown No.:l Concentration of standard Na2S&O3...
DATA SHEET Overall Stoichiometric Equations 1. Results and Observations 2. Unknown No.:l Concentration of standard Na2S&O3 solution, M 0.020(0 Trial 3 Trial 2 Trial 1 13.22 33.97 o.0.20 1311 Initial burette reading, mL 13.09 Final burette reading, mL (0.21 Volume of NAS2O3 used, mL Amount of NA2S2O3 used, mol Amount of Cu2 in unknown sample, mol L0.00 10. 00 10.00 Volume of original Cu solution used, mL in original solution, M Concentration of Cu Average Concentration of Cu2, M Procedure...
4.5e Question 1 Question 2 We now know the moles of hydroxide used in the titration....
4.5e Question 1
Question 2
We now know the moles of hydroxide used in the titration. But, remember that, of the 100 mL of solution made from the ashes, only 20 mL were used for the titration. What is the total amount of hydroxide produced by the ashes? Volume HCl used: 5.44 mlL Concentration HCI solution 0.10 M 5.44x 10 mol HCl used 5.44 x 104 mol OH in 20 ml. solution mol OH A 1.527-g sample of an unknown...
acid in a acne cleanser For each aliquot: 1) Use the calibration curve to determine the concentration of the salic...
acid in a acne cleanser For each aliquot: 1) Use the calibration curve to determine the concentration of the salicylic acid in the acne cleanser solution through interpolation of the absorbance measured for the acne cleanser sample, by using the equation of the line. 2 Calculate the moles and mass of salicylic acid present in the 25.00 mL volumetric flask. 3Y Determine the amount (mass) of salicylic acid in 1.00 mL of acne cleanser. 4) Using the density from the...
1) A standard Beer's law graph of complex lon concentration (2 SF) versus absorbance (3 SF) was prepared and...
1) A standard Beer's law graph of complex lon concentration (2 SF) versus absorbance (3 SF) was prepared and the equation describing the straight line is reported through Excel to be: A 11875 C (A: absorbance, C: concentration, M) Next, the following data was gathered in order to determine the Keq for the complex lon formation reaction. Calculate the value of K (6 pts) Stock sin [SCN'] 0.0023 M Stock sin (Fe") 0.0023 M A117750 Absorbance (complex ion) K11876 0103-11150...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a 500.0 ppm stock solution and diluting it to a final volume of 360.0 mL. Working standard 2 is prepared by taking 3.00 mL of working standard 1 and diluting it to a final volume of 250.0 mL. Calculate the ppm concentration of the working standard 2. Provide your answer to three places after the decimal and without units. Your Answer: Answer Question 5 (2...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
Question 1
Referring to Table 1 on page 9, what is the final concentration
of Fe+2 in ppm of the last standard solution
assuming that you used exactly the target value of 8.00 ml
Fe+2 in the 100 mL flask? For this question, assume that
you added exactly the volumes of all reagents as listed in the
table. (Remember that the stock solution concentration is 50.00
ppm.) Show all your work carefully
using good form and significant figures.
Table 1: Components...
THE DETERMINATION OF IRON BY SPECTROPHOTOMETRY INTRODUCTION In this experiment, the red-orange colored complex formed between iron(II) and 1,10- phenanthroline (Eqn.) is used in determination of iron by spectrophotometry. Fe+3PhenH Fe(Phen)2 +3H red-orange (A 512 nm) An excess of reducing reagent, such as hydroxylamine or hydroquinone, is often used to reduce and maintain iron in +2 oxidation state. The complex, once formed, is very stable, and can be stored for a long time. Required Reading: Skoog and West (9E): Chapter...
To determine the Ca^2+ concentration in water sample, a standard EDTA solution of 0.01988 M was used to titrate 25 ml. of the sample solution with the presence of an ammonium buffer (pH 10). If 15.80 ml. of the standard EDTA was used to reach the end point, calculate the molar concentration and the ppm concentration of the unknown Ca^2+ (atomic weight of Ca = 40.08g/mole) solution The amount of 0.2915g of benzoic acid was dissolved in 100 ml. of...
DATA SHEET Overall Stoichiometric Equations 1. Results and Observations 2. Unknown No.:l Concentration of standard Na2S&O3 solution, M 0.020(0 Trial 3 Trial 2 Trial 1 13.22 33.97 o.0.20 1311 Initial burette reading, mL 13.09 Final burette reading, mL (0.21 Volume of NAS2O3 used, mL Amount of NA2S2O3 used, mol Amount of Cu2 in unknown sample, mol L0.00 10. 00 10.00 Volume of original Cu solution used, mL in original solution, M Concentration of Cu Average Concentration of Cu2, M Procedure...
4.5e Question 1
Question 2
We now know the moles of hydroxide used in the titration. But, remember that, of the 100 mL of solution made from the ashes, only 20 mL were used for the titration. What is the total amount of hydroxide produced by the ashes? Volume HCl used: 5.44 mlL Concentration HCI solution 0.10 M 5.44x 10 mol HCl used 5.44 x 104 mol OH in 20 ml. solution mol OH A 1.527-g sample of an unknown...
acid in a acne cleanser For each aliquot: 1) Use the calibration curve to determine the concentration of the salicylic acid in the acne cleanser solution through interpolation of the absorbance measured for the acne cleanser sample, by using the equation of the line. 2 Calculate the moles and mass of salicylic acid present in the 25.00 mL volumetric flask. 3Y Determine the amount (mass) of salicylic acid in 1.00 mL of acne cleanser. 4) Using the density from the...
1) A standard Beer's law graph of complex lon concentration (2 SF) versus absorbance (3 SF) was prepared and the equation describing the straight line is reported through Excel to be: A 11875 C (A: absorbance, C: concentration, M) Next, the following data was gathered in order to determine the Keq for the complex lon formation reaction. Calculate the value of K (6 pts) Stock sin [SCN'] 0.0023 M Stock sin (Fe") 0.0023 M A117750 Absorbance (complex ion) K11876 0103-11150...
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