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1. The following chromatogram contained three samples before their development: sample A, sample B and sample...
Consider the following errors that could be made when running TLC. Indicate what should be done to correct the error. A two-component mixture containing 1-octene and 1, 4-dimethylbenzene gave only one spot with an Rf value of 0.95. The solvent used was acetone. A two-component mixture containing a dicarboxylic acid and tricarboxylic acid gave only one spot with an Rf value of 0.05. The solvent used was hexane. When a TLC plate was developed, the solvent front ran off the...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
1. which of the following are not absorbent for TLC? a. magnesium silicate b. polyamides c. silica gel-F ( fluorescing indicator added) d. starch e. alumina f. none of the above 2. select the best answer from the statements below about thin layer chromatography A. in normal phase thin layer chromatography the stationary phase is polar silica gel b. in normal phase thin layer chromatography the mobile phase is an organic solvent or a mixture of organic solvents Which is...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala plus lys. anion exchange resin was used Tubes 2,3, 6 and 7 had a spot appear that was light pink We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...