Homework Answers
The partition coefficient is the ratio of the concentration of the component in stationary and in the mobile phase. Also, partition coefficient is directly proportional to time. Therefore, highest partition coefficient is shown by species taking more time for eluting and vice-versa
1) At 8th min, species 1 already eluted, so the concentration of species 1 will be less than species 4. The first statement is wrong
2) Here, Species 4 has highest partition coefficient, as it is taking more time to elute. The second statement is correct
3) At 8th min, species 2 already eluted, so the concentration of species 2 will be less than species 4. The third statement is correct
4) The partition coefficient of 1, 2, 3 and 4 components are different. The fourth statement is wrong
5) Species 1 has lowest partition coefficient. The fifth statement is wrong
6) Species 4 has highest partition coefficient. The sixth statement is also wrong
7) Species 1 has lowest partition coefficient. The seventh statement is correct
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Species 1, 2, 3, and 4 were separated using gas chromatography (species 1 is the solvent)....
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a...
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a 2.00 m column having 5000 theoretical plates at a flow rate of 15.0 ml / min. The peak of unabsorbed air appears after 30 s; the peak of A appears after 5 min and that of B after 12 min (a) Calculate the dead volume VM of the column, and the retention volumes Va and VB. (b) Calculate the reduced volumes V’a and V'B...
3. There are two major components in the chromatography experiment: the solvent system (mobile phase) and...
3. There are two major components in the chromatography experiment: the solvent system (mobile phase) and the paper (stationary phase). Which of these major components is more polar phase and which is less polar phase? Explain using Lewis structures. (please PLEASE use the lewis structures, thats the part of the question I don't have yet!) 4. What parts of the molecules in the stationary phase will interact with the molecules that are being separated? Please answer this question using the...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In...
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L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of...
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1.In the following chromatogram, a mixture of pyridine (1), phenol (2), and toluene (3) was separated....
1.In the following chromatogram, a mixture of pyridine (1),
phenol (2), and toluene (3) was separated. Determine the retention
times of the components. Given this order of elution, would you
expect the stationary phase to be polar or non-polar?
Explain why the toluene peak is much shorter than the other two
peaks even though it is approximately the same concentration.
2. Stationary phases come in different varieties. Which of the
two analytes listed for each stationary phase has the greater...
PRELABORATORY ASSIGNMENT 1. Provide the key term that corresponds to each of the following definitions. (a)...
PRELABORATORY ASSIGNMENT 1. Provide the key term that corresponds to each of the following definitions. (a) the study of compounds derived from plants and animals (b) a method for separating a mixture into its components as a result of a varying attraction of compounds for a mobile solvent on a stationary solid (c) the leading edge of the solvent, which travels from the bottom of the developing chamber to the upper portion of the chromatogram (d) refers to solvent that...
Ethylene glycol boiling point-197.30C 1-butanol-117.6C 2-butanol-99.5 C Butanone-79.4 C How would a gradient elution(60...
Ethylene glycol boiling point-197.30C
1-butanol-117.6C
2-butanol-99.5 C
Butanone-79.4 C
How would a gradient elution(60 C-140 C) impact the
chromatogram?
Mobile Phase - Air (Flow Rate = 20 mL/min), Stationary Phase - poly(dimethylsiloxane), Oven Temperature - 75 °C. Ethane-1,2 diol (ethylene glycol), butanone, 1-butanol, 2-butanol In gas chromatography (GC) which component elute first depends on the boiling point. In GC the mixture is first converted to its gaseous states and then passes through the column. The component which has lowest boiling...
1. List two errors in the typical student set up for performing thin layer chromatography illustrated...
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Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent...
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1. Select an appropriate type of column for separating these steroids and give the rationale for...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a 2.00 m column having 5000 theoretical plates at a flow rate of 15.0 ml / min. The peak of unabsorbed air appears after 30 s; the peak of A appears after 5 min and that of B after 12 min (a) Calculate the dead volume VM of the column, and the retention volumes Va and VB. (b) Calculate the reduced volumes V’a and V'B...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of non polar solvent as the eluent in HPLC. (b) the use of polar material as the stationary phase in a chromatographic column. the use of non-polar material as the stationary phase in a chromatographic column 2) A solute with a retention time of 325 seconds has a base width of 15 seconds. The column is 11.5 cm long. The c (2 points) olumn has...
1.In the following chromatogram, a mixture of pyridine (1),
phenol (2), and toluene (3) was separated. Determine the retention
times of the components. Given this order of elution, would you
expect the stationary phase to be polar or non-polar?
Explain why the toluene peak is much shorter than the other two
peaks even though it is approximately the same concentration.
2. Stationary phases come in different varieties. Which of the
two analytes listed for each stationary phase has the greater...
PRELABORATORY ASSIGNMENT 1. Provide the key term that corresponds to each of the following definitions. (a) the study of compounds derived from plants and animals (b) a method for separating a mixture into its components as a result of a varying attraction of compounds for a mobile solvent on a stationary solid (c) the leading edge of the solvent, which travels from the bottom of the developing chamber to the upper portion of the chromatogram (d) refers to solvent that...
Ethylene glycol boiling point-197.30C
1-butanol-117.6C
2-butanol-99.5 C
Butanone-79.4 C
How would a gradient elution(60 C-140 C) impact the
chromatogram?
Mobile Phase - Air (Flow Rate = 20 mL/min), Stationary Phase - poly(dimethylsiloxane), Oven Temperature - 75 °C. Ethane-1,2 diol (ethylene glycol), butanone, 1-butanol, 2-butanol In gas chromatography (GC) which component elute first depends on the boiling point. In GC the mixture is first converted to its gaseous states and then passes through the column. The component which has lowest boiling...
1. List two errors in the typical student set up for performing thin layer chromatography illustrated here. 2. Define the following terms: Analyte: Stationary Phase: Mobile phase: Solvent Front: Origin: Rf Value: 3. Compound y traveled 6.2 cm while the solvent front traveled 11.1 cm. Calculate the Rf value for compound y. 4. Compound Z has an Rf value of 0725. When a chromatogram was developed, it moved 3.3 cm from the origin. How many cm did the solvent front...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
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