Using the Henderson-Hasselbalch equation, determine at what pH the ratio of amine to ammonium ion will be 1 to 100. Write your answer in the box provided.
I need to know how to get the answer.
pH = 7.6
Calculation:
The pH is calculated by following equation,
pH = pka + log [amine]/[ammonium ion]
From the given structure of amino acid,
The pka of ammonium ion = 9.6
The ratio of concentration of amine to ammonium ion = 1/100
Thus,
pH = 9.6 + log [amine]/[ammonium ion]
pH = 9.6 + log (1/100)
pH = 9.6 + log 0.01
pH = 9.6 - 2
pH = 7.6
Using the Henderson-Hasselbalch equation, determine at what pH the ratio of amine to ammonium ion will be 1 to 100. Write your answer in the box provided.
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