Question

Using the Henderson-Hasselbalch equation, determine at what pH the ratio of amine to ammonium ion will be 1 to 100. Write your answer in the box provided.

Using the Henderson-Hasselbalch equation, determine at what pH the ratio of amine to ammonium ion will be 1 to 100. Write your answer in the box provided.

I need to know how to get the answer.

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Answer #1

pH = 7.6

Calculation:

The pH is calculated by following equation,

pH = pka + log [amine]/[ammonium ion]

From the given structure of amino acid,

The pka of ammonium ion = 9.6

The ratio of concentration of amine to ammonium ion = 1/100

Thus,

pH = 9.6 + log [amine]/[ammonium ion]

pH = 9.6 + log (1/100)

pH = 9.6 + log 0.01

pH = 9.6 - 2

pH = 7.6


answered by: Allen
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