Question

3. The enzyme chymotrypsin is a protease that is competitively inhibited by a red dye. This protein and dye are combined in a solution and reach chemical equilibrium: protein + dye complex Prior to obtaining equilibrium and before any complexes have formed, the concentration of protein is G,o = 1.0 x 10-4 M and the concentration of dye is cd,o-2.0 x 10-3 M. (i) Write a simple expression that relates the equilibrium concentrations of protein cp and complex ce to the initial concentration of the protein, cp,o ii) Write an expression for the equilibrium concentration of the complex, ce in terms of the solution's absorbance A, the path length L, the concentrations cp, Cd, and cp,0, and the molar absorptivities of the three species, Ep, Ed, and Ec You measure an absorbance of the equilibrated solution at 280 nm of A 1.4 in a 1 cm cell. Also, at 280 nm, the molar absorptivities of the three different species are: species 280 [M11 protein dye complex 12000 0 15000

Answer 1

Date Page 4 Y, nats D)A ete po e