Question

Answer all the questions below The production of penicillin follows the bio reaction described below: C_6H_12O_6 + aO_2 + bNH_3 rightarrow cC_16H_20O_4N_2 + dH_2O + eCO_2 + fC_4H_7O_3N If the product yield Y_P/S was 0.25 g product/g substrate and 65% (wt/wt) of the carbon in the substrate is converted to biomass, calculate: The stoichiometric coefficients a, b, c, d, e and f. The amount of oxygen required to give cell concentration of 100 g/L (the reactor's volume is 5000 L). The degree of reduction of the substrate, biomass and product Additional information: Molecular weight of substrate: 180 Molecular weight of oxygen: 32 Molecular weight of carbon dioxide: 44 Molecular weight of product: 117 Molecular weight of biomass: 304 Molecular weight of ammonia: 17 Molecular weight of water: 18

Answer 1

The stoichiometric coefficients:

a = 5.845

b = 0.124

c = 0.062

d = 5.938

e = 1

f = 5

The degree of reduction of the substrate, biomass and product

$\gamma s = 4+12-2*6 = 4$

$\gamma b = 4+20-2*4-3*2=10$

$\gamma p = 4+7-2*3-3*1=2$