What wavelength would we use to perform normal phase HPLC on
these two compounds?
The common chromaophore that both the compounds have is at 214 nm. Therefore, that should have been the detection wavelength in UV detector
What wavelength would we use to perform normal phase HPLC on these two compounds? Ho oH
4) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C6H5CH2OH), benzaldehyde (C6H5CHO), benzoic acid (C6H5COOH), Acetophenone (C6H5C(O)CH3)
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00, circle which of the following compounds would elute first and briefly describe why: Propanoic acid (CH3CH2COOH, PK. = 4.87) or Propylamine (CH2CH2CH2NH2, pks = 3.43). b. If one uses gradient elution with normal phase chromatography, would it be better to go from a less polar to a more polar solvent or a more polar to a less polar solvent? Why?
4) (10 pts) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C.HSCH-OH), benzaldehyde (C6HSCHO), benzoic acid (C6HCOOH), Acetophenone (C6H5C(O)CH3)
25-17. (a) Nonpolar aromatic compounds were separated by HPLC on an octadecyl (CD bonded phase. The eluent was 65 vol% meth- anol in water. How would the retention times be affected if 90% methanol were used instead? (b) Octanoic acid and 1-aminooctane were passed through the same column described in (a), using an eluent of 20% metha- nol/80% buffer (pH 3.0). State which compound is expected to be eluted first and why Octanoic acid CH CH CH CH,CH CH,CH CH2NH2...
Which of the following compounds would have the lowest Rfvalue on a TLC plate? HO OH A. B. C. D. E.
x What is the relationship between e following compounds? HzC H OH, HzC OH HO H HO H CH OH CH OH Enantioners Diastereomers identical molecules geometrical Isomers
1) Circle all of the following compounds that is (are) primary alcohols он HO OH 2) Write the IUPAC of the following compound Но, 3) Write the common name of the following compound Vo 4) Circle the following compounds that has the highest boiling point + oH OH HOMOH 5) Circle which of the following would be a reasonable synthesis of 1-butanol но- H2O 1. BH/THE 2. H2O2. HO H2O H2O 1. BH/THF 2. H2O2, HO
How are the following two compounds related? CH CH3 HO -H HO -Н Given the following substitution reaction, what would the effect be of changing the solvent from CH3OH to (CH3)2S=0? H- OH H OH VS HO -H H -OH H -OH HO H + CH (CH2CH2-Br NaOH CH;(CH2), CH2-OH + NaBr CH OH CH2OH A. The reaction rate will increase. OB. There is no effect on the rate of the reaction, C. The reaction rate will decrease. Give the...
What is the order in which the following compounds (acetone, 1,2-dichloroethane, acetamide) would be eluted from an HPLC column containing a reversed-phase (RP) stationary? dichloroethane < acetone<acetamide acetamide <dichloroethane acetone acetone<acetamide <dichloroethane acetamide <acetone<dichloroethane
Which reaction conditions would you use to perform the reaction shown below? HO CHE O 1. BH3, THE 2. H2O2, OH, H20 O 1. Oso 2. NaHSO3 H2O 1.03 2. Zn/H20 or DMS 1. Hg(OAC)2, H20 2. NaBH4