Question

PreLab Questions: 1. Identify the stationary phase and the mobile phase in this lab 2. Compare and contrast the stationary phase and mobile phase 3. Identify safety precautions which should be taken while handling the stationary and mobile phases in this lab 4. Given the following paper chromatogram for 4 metal ions developed by a student in a previous semester. Calculate Rr value for each metal ion. Solvent o3 Front s.s 4,5 O- Point of Application 9.9

Doing A comparison

Answer 1

Solution

A) stationary phase - stationary phase is the solid or liquid supported on solid on which the mobile phase carringc solute moves.

Mobile phase - Is the phase which carries the solute and runnes on stationary phase. It can be liquid or gas not solid.

B) precautions while handling them is the stationary phase should be still and should be left undisturbed while mobile phase should be covered so that it won't evaporat while running.

C) Rf = retention factor = ( distance travelled by solute) / ( distance travelled by solution)

Distance travelled by solution = 6.3 cm

So a) Rf for first ion = 1.6/6.3 = 0.25

b) Rf for second ion = 2.9/6.3 = 0.46

C) Rf for third ion = 4.5/ 6.3 = 0.71

d) Rf for forth ion = 5.5/6.3 = 0.87