Question

ISI (HM (HM/min) 7. (pt A biochemist obtains the following set of data for an enzymes that is known to follow Michaclis-Menten kinetics. The total 49 96 349 621 676 698 699 I enzyme concentration was 1 nmol L 50 100 1000 5000 Explain how you a) Vma for the enzyme is determined this in 1 sentence. b) Km for the enzymes is . Explain how you determined this in 1 sentence ck(at for the enzyme is- . Show your calculations. 8. (pt) The turnover number (at) for an enzyme is known to beISI (HMv. (HM/min) 5000 min From the following data, calculate the K and the total amount of enzyme present in these experiments. Show your work or explain in detail how you came to these conclusions. 50 100 1000 5000 49 96 349 621 676 698 699

Answer 1

max = 699 tan/min Yaar maximum ma s As Kin Km we can col oulate t ulate to Kou