The above graph gives the absorbance of vitamin B, solution in water, in a 1.00 cm...
1. Did the column separate NADH and BSA? Explain based on your graph 2. What assumptions have you made in determining the absorbance of the mixture at two different wavelengths? 3. Is the reaction catalysed by GPT a reversible reaction? Give evidence from your experimental results in Table 3 to support your answer. (I DONT UNDERSTAND THIS QUESTION PLEASE HELP) Separation of BSA and NADH using Gel filtration chromatography 0.060 0.040 0.020 0.000 0.000 4.000 3.000 5.000 6.000 2.000 -0.020...