Question

How do I find diluted concentration(M)? Some examples would be great, thank you

Absorbance vs. Wavelength (A vs. A) Absorption Spectrum Peaks for the Diluted Grape-Flavored Drink Peak Amax Absorbance, A 507 nm 0.387 608 nm 0.528 1. Preparation Tables (9 p). Working with a stock solution of FD&C food dye Red #40 (3.55 x 10M) and another stock for FD&C food dye Blue #2 (3.79 x 10 M), you build two dilution sets using NANOpure water as diluent. Complete the following tables giving an example calculation. FD&C Food Dye Red #40 Dilution Set Preparation Volume of Stock (UL) Volume of Diluent (LL) Diluted Concentration (M) 5000 A at Amar = 507 nm 0.991 3750 1250 0.753 2500 2500 0.506 0.248 3750 1250 50 0.009 4950

Answer 1

S we shall use the formula visi = V2s2 for this problem. for Dye Red 40; [stock] = 3.55 x 1005 (N) 50000 = 3750 83.55x10-5 a) = 8.668 10-5 (M) 5000XS : 2500 x 3-55X10-5 s1795 X 10 -SIM) 5000 s 1250 x 3-55X10-5 =) S: 8.875 * 10+ (A) d) 5000rs = 50x3.55x10-5 =) sa 3.550 1007 (1) 61 for Dye Blue 2; [stock] = 3.79 x 10-5(M) geoest kes a 3. po-se os e 95 2x 1080) a 5x5= 3.75 X 3 7 9 X 10-5 = = 2.8425 x 10-5(M) 5x5 = 2.500 3.79 x 10-5=) = 1.845 8 10-SIM) 5x5= 1.253.74 x 10-5 =) 3 = 9.4A5 x 10-6(N) d) 5x5= 0.0 5x 3-79810-5 =) = 3.79 x 10-7 (M) Hope it helps. ☺