Species A and B were separated using column chromatography. The elution time for species A (tA) was 200 s with a peak width (wA) of 35 s, and the elution time for species B (tB) was 353 s with a peak width (wB) of 51 s. What is the length of the column (in centimeters) if the theoretical plate height for species B is 3.0 mm?
L=
Species A and B were separated using column chromatography. The elution time for species A (tA)...
20 > Species A and B were separated using column chromatography. The elution time for species A (A) was 200 s with a peak width (WA) of 35 s, and the elution time for species B (') was 382 s with a peak width (WR) of 42 s. What is the length of the column (in centimeters) if the theoretical plate height for species B is 1.0 mm? L =
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a 2.00 m column having 5000 theoretical plates at a flow rate of 15.0 ml / min. The peak of unabsorbed air appears after 30 s; the peak of A appears after 5 min and that of B after 12 min (a) Calculate the dead volume VM of the column, and the retention volumes Va and VB. (b) Calculate the reduced volumes V’a and V'B...
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on a 40.0 cm column Retention Compound time (min) Width at the base (min) A 5.17 0.436 B 1.02 13.0 15.0 C 1.10 23.4 1.91 Calculate the number of theoretical plates, N, for each peak. NA NB Nc ND = What is the average number of theoretical plates, Navg, for Navg the column? What is the plate height, H, for the column? Н-...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc,was found to be 207.6 s with a baseline peak width, wc, of 16.2 s. The retention time for aspartame, ta, was 253.7 s with a baseline peak width, wa, of 18.7 s. The retention time for the unretained solvent methanol was 40.7 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.3 cmlong...
Consider the data below for a liquid chromatography separation of four compounds, A, B, C, and D on a 45.0 cm column. Calculate the number of theoretical plates, N, for each peak. Retention Width at the Time (min) Base (min) 5.24 0.424 Number Number 12.8 1.08 NA = II 14.2 1.12 D 24.1 1.85 Number Number NB = 10 N= What is the average number of theoretical plates, Navg, for the column? Number Navy = avg What is the plate...
The following data applies to a column for liquid chromatography: Use this information to answer the question below Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture of species A, B, C, and D provided the following data: Species Retention time, minutes Width of peak base (W), minutes А 5.4 0.41 B 13.3 1.07 C 14.1 1.16 D 21.6 1.72 Calculate the resolution for species C and D Select one: O A 2.60...
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed with Chromosorb P and Carbowax 20M as stationary phase. The following retention times were obtained: air: 18.0 sec; methylacetate: 2.98 min; methyl propionate: 4.56 min. Peak width at half height: methylacetate: 7.7 sec; methyl propionate: 12.4 sec. Calculate: a) the corrected retention time for each solute. b) the capacity factor k' for each solute. c) the selectivity factor α for the pair of solutes....
need help with number 2 HW #3 for Chem 318 Due: April 29, 2019 1. Solute A has a K 2.5 for an extraction between water (phase 1) and chloroform (phase 2). If 100 mL of a 0.01 fraction will be extracted? For the same solute and, what fraction will be extracted if 5 extractions wi 80 mL chloroform each are used (instead of one 400 ml extraction)? M solution containing solute A in water is extracted one time with...