3) (30 pts) The small protein lysozyme is an important first line of defense against bacterial...
3) (30 pts) The small protein lysozyme is an important first line of defense against bacterial infection. It has 129 amino acids and contains both tyrosine and tryptophan. The absorbance of a 1 mg/ml sample of lysozyme was measured in a cuvette of thickness 1.00 cm and was found to be 3.19 at 240 nm and 2.58 at 280 nm. The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm3 mol-1 cm-1 and 1.12 x 104 dm3 mol-1 cm-1, respectively, and at 280 nm they are 5.40 x 103 dm3 mol-1 cm-1 and 1.50 x 103 dm3 mol-1 cm-1, respectively. What are the molar concentrations of the two amino acids in the 1 mg/ml protein sample? If the molar mass of the protein is 14,400 g/mol, how many tryptophans and tyrosines are present in the sequence of this protein? (looking for the molar ratio, moles aa/mol protein)