Question

Q. Briefly in point form or using a flow chart, describe what chromatographic techniques you

would use to completely separate a mixture of the 4 proteins listed below. After each part of

the techniques, BRIELFY outline what that particular technique accomplished (or show it in

your diagram).

Protein A: 35 kDa, pI = 3.5

Protein B: 35 kDa, pI = 8.5

Protein C: 100 kDa, pI = 8.5

Protein D: 100 kDa, pI = 3.5

Answer 1