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Immunology case study

There is a public debate as to why a passenger tests NEGATIVE with self-testing qualitative immunochromatography assay kit (cassette in) in a hotel room but tests POSITIVE with an Immunotechnique Assay called Immunofluorescence Assay (IFA) in an accredited laboratory upon arrival at the Ghana Kotoka International Airport. You’ve been asked to educate the Ghana Health Service and the general public 

a. Mention 5 factors that could validate the POSITIVE test results from the laboratory

b. Explain the principle behind both test assays.

c. Mention 3 practical precautions for RDT self-testing

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